Histological changes, Micronuclei induction and Nuclear abnormalities in the peripheral erythrocytes of Clarias gariepinus (Burchell 1822) exposed to water sample from Apodu Reservoir


Apodu reservoir
Clarias gariepinus
Water Pollution

How to Cite

Anifowoshe, A., Oyebanji, J., Oladipo, O., Oyeyemi, F., Abdulrahim , M., Abdulkareem, S., & Mustapha, M. (2020). Histological changes, Micronuclei induction and Nuclear abnormalities in the peripheral erythrocytes of Clarias gariepinus (Burchell 1822) exposed to water sample from Apodu Reservoir. Journal of Life and Bio Sciences Research , 1(01), 01-07. https://doi.org/10.38094/jlbsr113


Pollution of water bodies in most developing countries is of grave concern since toxic pollutants interact with the genetic blueprints of aquatic organisms leading to a variety of illnesses. In this study, histological changes in the gills, liver and kidney as well as the genotoxic effect of water sample from Apodu reservoir, were investigated in the peripheral erythrocytes of cultured catfish (Clarias gariepinus) using micronucleus assay. They were exposed to the water sample for a period of 3, 7 and 14 days. The physico-chemical (pH, temperature, transparency) and heavy metals analysed (Ca>Mg>Fe>Zn>Mn>Cu>Ni=Pb=Cd=Cr) were all within the acceptable limit permissible. However, the micronucleus test shows statistically significant (p<0.05) induction of micronuclei and nuclear abnormalities as compared to the control (borehole water). The histopathological analysis shows some mild alterations in the organs such as distortion and infiltration of lymphocytic cells of the gills. We also reported mild distortion of hepatic tissue of the liver as well as abnormal nephron and glomeruli with congestion and vascular dilation of the kidney. These alterations might be due to anthropogenic activities that pollute the water body. Therefore, the influx of agricultural and domestic waste into the Apodu reservoir should be discouraged as part of remediating efforts.



Aanand, S., Purushothaman, S., Pal, A.K., Rajendran, K.V. (2012). Toxicological studies on the effect of copper, lead and zinc on selected enzymes in the adductor. Mutation Research, 746: 7– 14.

Adeyeye, E. (1996). Determination of major elements in Illisha africana fish, associated water and soil sediments from some freshwater ponds. Bangladesh Journal of Scientific and Industrial Research, 31: 171-184.

Adoni, A.D. (1985). Work Book on Limnology. Bandna Printing Service, New Delhi. 216 pp.

Al-Sabti, K. (1991). Handbook on genotoxic effects and fish chromosome. Jozef Stefan Institute, Jamova.

Al-Sabti K., Metcalfe C.D. (1995). Fish micronuclei for assessing genotoxicity in water. Mutation Research, 343: 121-135.

Alimba, C.G., Saliu, J.K., Adesanya, A., and Bakare, A.A. (2011). Evaluation of Genotoxicity of municipal landfill leachate by micronucleus test using Clarias gariepinus. Research in Environment and Life Sciences, 4: 1–6.

Anifowoshe, A.T., Oladipo, S.O., Owolodun, O.A., Akinseye, K.M., Olafimihan, T.F., Sidiq, A.G., Muhammed, A.A., Asa, T.A., Mustapha, M.K. (2018). Eco-genotoxicological assessments of some selected fish species from Apodu Reservoir, Malete, North Central, Nigeria. Manila Journal of Science. (11),1-14.

Anifowoshe, A.T., Oladipo, O.S., Adebayo, M.O., Eboh, S.O., Abdussalam, R.A., Adegbenro, M.A., Ojo, I.T., Adetunji, K.A., Yekeen, H.O., Adebiyi, K.A., Tijani, S.O., Oladipupo, R.O. Abdulkareem, S.I. (2019). Induction of Micronuclei, Base-pair Substitution Mutation and Excision-repair Deficient by Polluted Water from Asa River in Nigeria. Annal of Science Technology, 4 (2) 68-77.

Bakare, A.A., Alabi, O. A., Gbadebo, A.M., Ogunsuyi, O.I., Alimba, C.G. (2013). In Vivo Cytogenotoxicity and Oxidative Stress Induced by Electronic Waste Leachate and Contaminated Well Water. Challenges, 4, 169-187.

Barsiene, J., Dedonyte, V., Rybakovas, A., Andreikenaite, L., Andersen, O.K. (2006). Investigation of micronuclei and other nuclear abnormalities in peripheral blood and kidney of marine fish treated with crude oil. Aquatic Toxicology, 78, S99–S104.

Boyd, C.E. (1990). Water Quality in Ponds for Aquaculture, Birmingham Publishing Co., Birmingham, Alabama.

Carrasco, K.R., Tilbury, K.L., Myers, M.S. (1990). Assessment of the piscine micronucleus test as in situ biological indicator of chemical contaminant effects. Canadian Journal of Fisheries and Aquatic Sciences, 47: 2123–2136.

Camargo, M.M.P., Martinez, C.B.R. (2007). Histopathology of gills, kidney and liver of a Neotropical fish caged in an urban stream. Neotropical Ichthyology, 5(3), 327-336.

Cavas, T. (2008). In vivo genotoxicity of mercury chloride and lead acetate: Micronucleus test on acridine orange stained fish cells. Food and Chemical. Toxicology, 46, 352–358.

Cavas, T., Ergene-Gozukara, S. (2003). Evaluation of the genotoxic potential of lambda– cyhalothrin using nuclear and nucleolar biomarkers on fish cells. Mutation Research, 534: 93–99.

Cladwell, C.A. (1997). Aromatic hydrocarbon pathology in fish following a large spill into the Nemadji river, Wisconsin, USA. Bullentin of Environmental Contamination and Toxicology, 58: 574-581.

Da Silva Souza, T., Fontanetti, C.S. (2006). Micronucleus test and observation of nuclear alterations in erythrocytes of Nile tilapia exposed to waters affected by refinery effluent, Mutation Research, 605 (1–2), 87–93.

De Flora, S., Vigario, L., D'Agostini, F., Camoirano, A., Bagnasco, M., Bennecelli, C., Arillo, A. (1993). Multiple biomarkers in fish exposed in situ to polluted river water. Mutation Research, 319: 167-177.

Delemos, C.T., Rodel, P.M. Terra, N.R., Erdtmann, B. (2007). Evaluation of basal micronucleus frequency and hexavalent chromium effects in fish erythrocytes. Environmental Toxicology and Chemistry, 20, 1320–1324.

EDRS (2016). Retrieved from www.domesticboreholes.co.za/the-top-benefits-of-borehole-water/

EFSA, (2005). Opinion of the Scientific Panel on contaminants in the food chain [CONTAM] related to the safety assessment of wild & farmed fish. Retrived fromhttp://www.efsa.europa.eu/EFSA/efsa_locale-1178620753812_ 1178620762697 .htm.

Ebenezer M. (2003). A comparative study of heavy metals in the water of an urban and rural pond. Journal of Ecobiology 15 (6), 407-412.

Ergene, S., Cavas, T., Celik, A., Koleli, N., Aymak, C. (2007). Evaluation of river water genotoxicity using piscine micronucleus test. Environmental and Molecular Mutagenesis, 48: 421–429.

Jana, S., Bandopadhyaya, N. (1987). Effect of heavy metals on some biochemical parameters in the freshwater fish, Clarias punctatus. Environment and Ecology, 5(3), 488-493.

Javed, M., Usmani, N. (2011). Accumulation of heavy metals in fishes: a human health concern. International Journal of Environmental Sciences, 2:659–670.

Keskin, T. E. (2010). Nitrate and heavy metal pollution resulting from agricultural activity: a case study from Eskipazar (Karabuk, Turkey). Environmental Earth Sciences. 61:703–721.

Lam, P.K.S., Gray, J.S. (2003). The Use of Biomarkers in Environmental Monitoring Programmes. Marine Pollution Bulletin, 46: 182-186.

Mayon, N., Bertrand, A., Leroy, D., Malbrouck, C., Mandiki, S.N.M., Silvestre, F., Kestemont, P. (2006). Multiscale approach of fish responses to different types of environmental contaminations: a case study. Science Total Environment, 367: 715–731.

Mitchell, S., Kennedy, S. (1992). Tissue concentrations of organochlorine compounds in common seals from the coast of Northern Ireland. Science Total Environment, 115: 235-240.

Osman, A.G.M., Abuel-Fadl, K.Y., Kloas, W. (2012). In situ evaluation of the genotoxic potential of the river Nile: II. Detection of DNA strand-breakage and apoptosis in Oreochromis niloticus niloticus (Linnaeus, 1758) and Clarias gariepinus (Burchell,1822). Mutation Research, 747: 14–21.

Ozkan, F., Gündüz, S.G., Berköz, M. Hunt, A.O. (2011). Induction of micronuclei and other nuclear abnormalities in peripheral erythrocytes of Nile tilapia, Oreochromis niloticus, following exposure to sublethal cadmium doses. Turkish Journal of Zoology, 35: 585–592.

Pacheco M., Santos M. A. (2002). Biotransformation, genotoxic and histopathological effects of environmental contaminants in European eel, Anguilla anguilla L. Ecotoxicology and Environmental Safety, 53, 331-347.

Pantaleao, S.M., Alcantara, A.V., Alves, J.P., Spano, M.A. (2006). The piscine micronucleus test to assess the impact of pollution on the Japaratuba river in Brazil. Environmental and Molecular Mutagenesis, 47(3), 219-224.

Park, E., Lee, J., Etoh, H. (1993). Fish Cell line (ULF-23HU) Derived from the Fin of the Central Mudminnow (Umbra limi): Suitable Characteristics for Clastogenicity Assay. In Vitro Cellularand Developmental Biology, 25: 987- 994.

Saotome, K., Hayashi, M. (2003). Application of a sea urchin micronucleus assay to monitoring aquatic pollution: influence of sample osmolality. Mutagenesis. 18(1): 73-6.

Sarangi, P.K., Patnaik R, Porichha S.K., Prasad R. (2001). Genotoxicity of malathion in Channa Punctatus cultured in vivo Perspective in Cytology and Genetics, 10, 835-844 (Editors G.K.Manna and S. C. Roy, AICCG Publication, Kalyani University.

Rodilla, V. (1993). Origin and evolution of binucleated cells and binucleated cell with micronuclei in cisplatin—Treated CHO cultures. Mutation Research, 300: 281–291.

Rybakovas, A., Barsiene, J., Lang, T. (2009): Environmental Genotoxicity and Cytotoxicity in Offshore Zones of the Baltic and North Seas. Marine Environmental Research, 68: 246-256.

WHO (2006). Guideline for Drinking Water quality (electronic Resource). Incorporating first Addendum 1, Recommendations 3rd retrieved from http://www.whglibdoc.who.int/publications/2006/9241546- 964.eng.pd.

United States Environmental Protection Agency (USEPA) (1996): Acid digestion of sediments, sludges and soils, Method-3050B.USEPA, Washingtn, DC.

Ventura, B.C., Angelis, D.F., Marin-Morales, M.A. (2008): Mutagenic and genotoxic effects of the atrazine herbicide in Oreochromis niloticus (Perciformes, Cichlidae) detected by the micronuclei test and the comet assay. Pesticide Biochemistry and Physiology, 90, 42–51.

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