The study was carried out on (120) various clinical samples collected from patients attended Al Salam and Al-Khansa Hospitals and the Public Health Laboratory in the Mosul city during the of 4 months (September - December 2019). Samples were cultured on Mannitol Salt Agar medium, 105 samples give a positive result (grew the bacteria). 50 isolates were fermented mannitol sugar, at a rate of 47.6%, depending on the phenotypic characteristics and production of Coagulase, 14 isolates were identified, at a rate of 13.3%, belonging to Staphylococcus aureus the diagnosis were confirmed using VITEK system. The highest isolation rate from wounds was 57%, then abscesses 21%, blood samples 14%, and urinary tract infections 7%. The sensitivity of the isolates was tested for 16 antibiotics, the isolates showed variation in their resistance to antibiotics. Most of the isolates showed high resistance at 92.8% to each of Oxacillin, Vancomycin, which were diagnosed as MRSA and VRSA. Vancomycin MICs against MRSA and VRSA ranged (2500-5000) g /mL. MIC for nanoparticles sized (30, 20, 50-150) nm ranged (5000-10000) g / mL for isolates that are positive coagulase. In this study the efficacy of vancomycin was improved in combination with ZnO nanoparticles. results showed a decrease in vancomycin MICs from (2500-5000) g / mL to (39-78.125) g \ mL when mixed with ZnO 20 nm.
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