Protozoans of Entamoeba spp. are globally distributed protozoan parasites that infect diverse hosts (human and animals) causing amebiasis with various symptoms ranging from abdominal discomfort, indigestion, diarrhoea, bloody diarrhoea, and even death. Entamoeba histolytica infection may be influenced by different strains which are already existing in our population. A meta-analysis was performed to evaluate the sequence comparison and geneflow of E. histolytica in Iraq and Iran. For this purpose, all reference sequences recorded from the aforementioned countries and deposited in the National Centre for Biology Information (NCBI) database of the mentioned countries (133 reference sequences, 110 from Iraq and 23 from Iran) were included in this study. After aligning and blasting all these sequences and considering the shared regions, eight unique sequences were obtained. According to the Codon-based Z-test of selections, they vary in degree of difference (p-value 0.05). Some records from the study area approached each other 100% which means that geneflow has occurred in the areas under investigation. Outstanding phylogenetic relationship of Entamoeba histolytica of both Iraq and Iran strains are related together and that is important in the molecular epidemiology aspect of amebiasis as it may influence the clinical and pharmacological orientation of the disease in both countries. Current meta-analysis was done for the first time in that approach in the place this study.
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